Use of the Flp-In™ System to generate stable expression cell lines provides a number of advantages as described below:
An expression plasmid containing a FRT site linked to the hygromycin resistance gene for Flp recombinase-mediated integration and selection of a stable cell line expressing your gene of interest under the control of the human cytomegalovirus (CMV) immediate-early enhancer/promoter.A Flp-In™ target site vector, pFRT/lacZeo, for generation of a host cell line containing an integrated FRT site.The major components of the Flp-In™ System include: An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase-mediated DNA recombination at the FRT site (O'Gorman et al., 1991).
The Flp-In™ System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice. The Flp-In™ System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location.
